Regenerative Dentistry: PRF in Clinical Practice

– Gingival recession treatment with PRF
– CTG harvesting techniques
– Intrabony defect management with PRF
– Socket healing after extraction
– Utilization of PRF in implant dentistry
– Sinus lift procedures with PRF
– PRF applications in wound healing
– Bio-PRF grafts for tissue regeneration
– Clinical case analysis.

– Bio-PRF:

- Vascular Endothelial Growth Factor (VEGF)
- Platelet-Derived Growth Factor (PDGF)
- Transforming Growth Factor-β (TGF-β).

– Fundamental principles of PRF
– PRF preparation methods
– Clinical techniques for PRF harvesting and its biological properties
– Influence of gender, age, and time on PRF
– Literature review and research analysis of PRF
– Injectable PRF (i-PRF)
– Fixed angle vs. horizontal centrifugation.

– Class of platelet concentrate
– Leukocyte and platelet-rich fibrin
– Simplicity of PRF protocols
– The "Low-Speed" centrifugation concept
– Spin protocols
– Horizontal centrifugation of PRF.

– Analyzing the properties of blood cells
– Advantages of horizontal centrifugation
– Fixed angle vs horizontal centrifuges
– Understanding the role of blood cells in platelet concentrate formation
– Comparison of yield and quality between fixed angle and horizontal centrifuges
– Exploring the impact of centrifugation speed and time on PRF properties.

– Setup and preparation
– Loading samples into fixed angle centrifuge
– Loading samples into horizontal centrifuge
– Operation and observation of the centrifugation process.

– Understanding G-force
– 10ml subs vs. 13ml
– Analysis of plasma volume;
– Yield and concentration of platelets
– 700g and 1000g protocol
– 100g and 200g protocol
– The 3 protocols for Bio-PRF
– Concentrated PRF (C-PRF)
– I-PRF vs. C-PRF.